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Figure 6 | Stem Cell Research & Therapy

Figure 6

From: Decellularized silk fibroin scaffold primed with adipose mesenchymal stromal cells improves wound healing in diabetic mice

Figure 6

Ad-MSCs-SF and D-Ad-MSCs-SF stimulate outgrowth of microvessels in the aorta ring assay. Rat dorsal aorta was excised and around 1 mm-thick rings were prepared. The rings were placed into 40 uL of collagen solution and incubated at 37°C for 30 minutes to obtain collagen jellification. At the end of incubation of HUVECs with control medium or SF, Ad-MSCs-SF- and D-Ad-MSCs-SF-derived CM were added. In A) the quantification of neovessel outgrowth from aortic rings obtained by counting under an inverted microscope at 10× magnifications after 10 days of incubation is reported. Bars are means ± SD of number of neovessel formation. The assay was repeated twice and each sample was run in triplicate. *P <0.05; **P <0.01 versus rings cultured in the presence of CM derived from HUVECs treated with control SF patches. Photographs of capillary outgrowth from aorta rings were taken under the different treatment conditions B). Ad-MSCs-SF, silk fibroin patch cellularized with human adipose-derived mesenchymal stromal cells; CM, conditioned medium; D-Ad-MSCs-SF, silk fibroin patch after human adipose-derived mesenchymal stromal cells removal (decellularization); HUVEC, human umbilical vein endothelial cells; SF, silk fibroin.

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