Figure 2

Human cadaver mesenchymal stromal/stem cell phenotypic and molecular characterization. (A) Representative flow cytometry analysis of mesenchymal, pericytic, stem cell, hematopoietic and vascular markers. Isotype controls are presented as filled black histograms, the specific cell markers as white histograms. (B) Flow cytometry analysis of hematopoietic, mesenchymal and pericyte marker coexpressions. Percentage and cytograms from a representative experiment. Immunofluorescence staining for Vimentin (C) and Nestin (D) in human cadaver mesenchymal stromal/stem cells (hC-MSCs). Nuclei were counterstained with DAPI (blue) and cell positive in green. Scale bars = 100 μm. (E) Stem cell gene expression analysis of SOX2, c-KIT, OCT-4, NOTCH-1 and KDR. β-Microglobulin was used as the housekeeping gene. The far left lane contains a 100 base pair ladder.