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Figure 1 | Stem Cell Research & Therapy

Figure 1

From: Towards an advanced therapy medicinal product based on mesenchymal stromal cells isolated from the umbilical cord tissue: quality and safety data

Figure 1

Isolation of UCX® cells . A standard protocol using a mixture of crude collagenase and porcine trypsin for cell isolation (ENZ(RG)), and fetal bovine serum (FBS) for cell culture was adapted by replacing the digestion enzymes with clinical grade enzymes (ENZ(CG)) and culturing the cells in human serum (HS) or human Platelet Lysate (hPL). The efficiency of the process was plotted taking into consideration the total cells at the end of passage 0 (P0) isolated from a standard cord (17.5 g, considering an average weight of 1 gram per centimeter of cord), and the days to confluence. Results are represented as mean ± SEM.

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