Figure 5

Immunohistologic analyses. Representative organ sections, after intracoronary injection of BM-MNCs labeled prior to injection with CFSE (green fluorescence). Cell nuclei were labeled with propidium iodide (red fluorescence). A: Fluorescent artefacts within the necrotic area of infarcted tissue. B-C: Myocardium sections of infarcted tissue, in absence of necrotic area, one hour after CSFE+ BM-MNC intracoronary injection. D: Myocardium section of a control animal (with myocardial infarction, without BM-MNC injection). E-F: The following section of C was stained with HES. Engrafted BM-MNCs within the infarcted zone appeared as round and dense blue points (arrow), in contrast to the elongated and blue pale cardiomyocyte nuclei (star). G: Myocardium section stained for endothelial cells and capillaries using anti-VonWillebrand antibody (red fluorescence). H-I: Lung (H) and liver (I) section. Cell nuclei labeled with propidium iodide (red fluorescence). J: Myocardium section of infarcted tissue, 24 hours after CFSE+ BM-MNC intracoronary injection. K-L: Myocardium section of an animal without myocardial infarction, one hour after CFSE+ BM-MNC intracoronary injection, using VonWillebrand antibody (K) or HES coloration (L).