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Figure 3 | Stem Cell Research & Therapy

Figure 3

From: Human adipose-derived stem cell transplantation as a potential therapy for collagen VI-related congenital muscular dystrophy

Figure 3

Histochemical and indirect immunofluorescence analyses of GCM biopsies after transplantation of human neonatal ADSC into Col6a1−/−Rag1−/−mice. Muscle biopsies were collected at one, two, three, four, and six weeks after transplantation under homeostatic conditions. Time points are indicated to the left of the panels. (A) Hematoxylin and eosin staining (H & E) of the ADSC-treated GCM. (B, C) Indirect immunofluorescence detection of ADSC-derived collagen VI in the transplanted GCM. Donor cells were detected with anti-human lamin A/C antibodies (AlexaFluor488, green) and collagen VI-positive myofibers were detected with anti-α1(VI)-collagen antibodies (AlexaFluor594, red). Images were taken from representative sections at low (B) and high (C) magnification, respectively. (D, E) Co-localization of the ADSC-donated α1(VI)-collagen (AlexaFluor488, green) and basement-membrane-associated type IV collagen (AlexaFluor594, red) in ADSC-treated muscles. Images were taken from representative sections at low (D) and high (E) magnification, respectively. Nuclei were stained with DAPI (blue). Scale bar, 100 μm (low magnification) and 25 μm (high magnification), respectively. ADSC, adipose-derived stem cells; DAPI, 4′,6-diamidino-2-phenyl indol; GCM, gastrocnemius muscle.

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