Figure 4

Analysis of muscle tissue infiltration with CD11b-positive cells after ADSC transplantation. (A) Indirect immunofluorescence assessment of the infiltrating CD11b-positive leukocytes (green) in untreated and ADSC-treated muscles of Col6a1^−/−Rag1^−/− mice at different time points after transplantation. (B) Indirect immunofluorescence assessment of the infiltrating CD11b-positive leukocytes (green) in CTX-injured muscles with or without ADSC transplantation. In all cases, ADSC were detected with anti-human lamin A/C antibodies (red), whereas infiltrating CD11b-positive cells were detected with anti-CD11b antibodies (green). Time points are indicated to the left of the panels. Corresponding treatment is shown on top of the panels. Nuclei were stained with DAPI (blue). Scale bar, 100 μm. ADSC, adipose-derived stem cells; CTX, cardiotoxin; DAPI, 4′,6-diamidino-2-phenyl indol.