Figure 6
Comparative morphometric analysis of ADSC engraftment and type VI collagen distribution in homeostatic and CTX-treated GCM, respectively. Analysis was performed at one, two, three, four and six weeks post-transplantation and CTX injury. (A) Engraftment of ADSC into the GCM with or without CTX treatment. The vertical axis shows the percentage (%) of engrafted ADSC per injected muscle. The horizontal axis shows the time point after transplantation in weeks (w). (B) Morphometric analysis of α1(VI)-positive myofibers in the transplanted GCM with or without CTX treatment. The vertical axis shows the number of α1(VI)-positive myofibers per section. The horizontal axis shows the time point after transplantation in weeks (w). In all cases, ADSC were detected with anti-lamin A/C antibodies. The percentage of engrafted cells and the number of α1(VI)-positive myofibers were determined on at least fifty 7 μm cryosections, respectively, covering approximately 125 mm3 of muscle tissue. In all studies, at least five animals were analyzed per time point. Data is presented as average ± SD. ADSC, adipose-derived stem cells; CTX, cardiotoxin; GCM, gastrocnemius muscle; SD, standard deviation.