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Table 1 Differential expression analysis in primary human neonatal ADSC

From: Human adipose-derived stem cell transplantation as a potential therapy for collagen VI-related congenital muscular dystrophy

Gene symbol RefSeq number Description Signal Fold difference gene/GAPDHa
Extracellular matrix proteins
Collagens and ECM structural constituents
COL1A1 NM_000088 Homo sapiens collagen, type I, alpha 1 183526 2.06
COL1A2 NM_000089 Homo sapiens collagen, type I, alpha 2 142138 1.59
COL3A1 NM_000090 Homo sapiens collagen, type III, alpha 1 3108 0.03
COL4A1 NM_001845 Homo sapiens collagen, type IV, alpha 1 5622 0.06
COL4A2 NM_001846 Homo sapiens collagen, type IV, alpha 2 44164 0.49
COL5A1 NM_000093 Homo sapiens collagen, type V, alpha 1 32129 0.36
COL5A2 NM_000393 Homo sapiens collagen, type V, alpha 2 19919 0.22
COL6A1 NM_001848 Homo sapiens collagen, type VI, alpha 1 117011 1.31
COL6A2 NM_058174 Homo sapiens collagen, type VI, alpha 2 11593 0.13
COL6A3 NM_004369 Homo sapiens collagen, type VI, alpha 3 1112 0.01
COL7A1 NM_000094 Homo sapiens collagen, type VII, alpha 1 1136 0.01
COL12A1 NM_004370 Homo sapiens collagen, type XII, alpha 1 17353 0.19
COL16A1 NM_001856 Homo sapiens collagen, type XVI, alpha 1 17366 0.19
DCN NM_001920 Homo sapiens decorin 22826 0.25
FN1 NM_054034 Homo sapiens fibronectin 1 6213 0.07
LUM NM_002345 Homo sapiens lumican 7747 0.09
Basement membrane constituents
LAMA2 NM_000426 Homo sapiens laminin, alpha 2 804 0.01
LAMB1 NM_002291 Homo sapiens laminin, beta 1 10371 0.11
LAMB2 NM_002292 Homo sapiens laminin, beta 2 (laminin S) 16172 0.18
LAMC1 NM_002293 Homo sapiens laminin, gamma 1 (formerly LAMB2) 10072 0.11
Cell adhesion molecules
Cell-matrix adhesion
ITGA5 NM_002205 Homo sapiens integrin, alpha 5 (fibronectin receptor, alpha polypeptide) 3324 0.04
ITGA6 NM_000210 Homo sapiens integrin, alpha 6 1168 0.01
ITGA7 NM_002206 Homo sapiens integrin, alpha 7 7553 0.08
ITGB1 NM_133376 Homo sapiens integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12) 6616 0.07
ITGB5 NM_002213 Homo sapiens integrin, beta 5 4324 0.05
ECM proteases and protease inhibitors
MMP1 NM_002421 Homo sapiens matrix metallopeptidase 1 (interstitial collagenase) 4368 0.05
TIMP1 NM_003254 Homo sapiens TIMP metallopeptidase inhibitor 1 42809 0.48
TIMP2 NM_003255 Homo sapiens TIMP metallopeptidase inhibitor 2 83481 0.93
TIMP3 NM_000362 Homo sapiens TIMP metallopeptidase inhibitor 3 36463 0.41
Non-muscle and muscle markers
DES NM_001927 Homo sapiens desmin 8119 0.09
MYO1C NM_033375 Homo sapiens myosin IC 33956 0.38
MYLK NM_053025 Homo sapiens myosin light chain kinase 12346 0.14
MYLK NM_053025 Homo sapiens myosin light chain kinase 18846 0.21
MYH9 NM_002473 Homo sapiens myosin, heavy chain 9, non-muscle 15884 0.18
MYL6 NM_079423 Homo sapiens myosin, light chain 6, alkali, smooth muscle and non-muscle 51777 0.58
  1. aThe fold difference represents the ratio of intensity of each gene hybridized with the RNA isolated from hADSC normalized to the intensity of GAPDH gene. Each array was processed in an identical manner and the number represents an average of triplicate experiments from three independent cell isolates. Each gene is demarcated by the Genbank accession number, the description of the gene and the common name. Genes are grouped by their distinct functional categories. ADSC, adipose-derived stem cells; ECM, extracellular matrix.