Figure 5

Migration of exogenous cell types in response to embryoid body conditioned medium. (A) Fibroblast migration was stimulated by embryoid body (EB) conditioned medium (CM) collected from different days of differentiation compared with unconditioned medium (uCM) up to 12 hours, but at 24 hours only CM at day 9 (CM 9) induced greater cell migration compared with uCM. (B) After 24 hours, EB-CM promoted the formation of fibroblast network structures compared with cells treated with uCM, which remained more as single cells. (C) Endothelial cell migration was stimulated by all of the EB-CM compositions compared with uCM. By 24 hours, CM at day 12 (CM 12) induced significantly more cells to migrate compared with CM at day 6 (CM 6) and CM 9. (D) After 24 hours, endothelial cells treated with EB-CM prompted cells to cluster whereas the uCM did not influence the organization of endothelial cells. Results indicate the mean ± standard error (n = 6). Analysis of variance: *P < 0.05 compared with all EB-CM time points; #P < 0.05 compared with CM 6 and CM 9; †P < 0.05 compared with CM 9. HUVEC, human umbilical vein endothelial cell.