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Table 1 Primer nucleotide sequences

From: Soluble factors secreted by differentiating embryonic stem cells stimulate exogenous cell proliferation and migration

Primer

Nucleotide sequence (5′ to 3′)

Gapdh

Forward

GCC TTC CGT GTT CCT ACC

Reverse

GCC TGC TTC ACC ACC TTC

Oct-4

Forward

CCG TGT GAG GTG GAG TCT GGA G

Reverse

GCG ATG TGA GTG ATC TGC TGT AGG

Afp

Forward

CAC ACC CGC TTC CCT CAT CC

Reverse

TTC TTC TCC GTC ACG CAC TGG

Bmp-4

Forward

CTG GCC CGG AAG CTA GGT GAG TT

Reverse

GAG GGC CAG AGA CTG GAT CGC

Fgf-2

Forward

AGC GAC CCA CAC GTC AAA CTA C

Reverse

CAG CCG TCC ATC TTC CTT CAT A

Igf-1

Forward

TCC GCC AGG TTG CCT CTA G

Reverse

GGA AGC AGG TGG ATG GTC AG

Igf-2

Forward

TCA AAG AGT TCA GAG AGG

Reverse

CAA CCA TCA GTG AAT CAA A

Pdgfb

Forward

ATC GCC GAG TGC AAG ACG CG

Reverse

AAG CAC CAT TGG CCG TCC GA

Vegfa

Forward

TGC ACC CAC GAC AGA AGG

Reverse

GCA CAC AGG ACG GCT TGA

  1. Forward and reverse primer nucleotide sequences for the polymerase chain reaction amplification of glyceraldehyde-3-phosphate dehydrogenase (Gapdh), Octamer-4 (Oct-4), α-fetoprotein (Afp), bone morphogenetic protein-4 (Bmp-4), fibroblast growth factor-2 (Fgf-2), insulin-like growth factor-1 and factor-2 (Igf-1, Igf-2), platelet-derived growth factor-B chain (Pdgf-b), and vascular endothelial growth factor-A (Vegfa). All reverse transcriptase-polymerase chain reactions performed with an annealing temperature of 58°C.
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Stem Cell Research & Therapy

ISSN: 1757-6512