Role of PLZF on MSC differentiation. (A) Effect of PLZF overexpression on MSC chondrogenic differentiation after 21 days in micropellet culture. Expression of Sox9 and the cartilage marker Col2A1 in C3 and C3-PLZF, cells assessed by RT-qPCR and immunohistochemical analysis for collagen II expression in pellets. (B) Effect of PLZF overexpression on MSC adipogenic potential. Expression level of PPAR-γ and the adipocyte marker (FABP4) determined by RT-qPCR and staining of lipid droplets with oil red O. (C) Effect of PLZF overexpression on MSC osteogenic potential. Expression level of Runx2 and the osteoblast marker osteocalcin (OC), determined by RT-qPCR, and alizarin red S staining for mineralization. RT-qPCR data represent the mean ± SEM of three independent experiments.