Figure 2
From: Human Wharton’s jelly mesenchymal stem cells promote skin wound healing through paracrine signaling
Wharton's jelly-derived mesenchymal stem cell conditioned medium increases normal skin fibroblast proliferation, but does not affect apoptosis. Cell proliferation was examined using Ki67 staining. (A) Wharton's jelly-derived mesenchymal stem cell conditioned medium (WJ-MSC-CM)-treated normal fibroblasts showed enhanced proliferative rates compared with the control group (quantified in (B)). (C) Terminal transferase TdT-mediated dUTP biotin end-labeling (TUNEL) staining of WJ-MSC-CM-treated human normal skin fibroblasts versus control (non-WJ-MSC-CM-treated) normal skin fibroblasts showed no significant difference in induction of apoptosis (quantified in (E)). Note that the total number of viable cells was significantly higher in the WJ-MSC-CM-treated cells compared with the non-WJ-MSC-CM-treated cells (D). n = 3 samples per group. *P ≤ 0.05, ***P ≤ 0.001. DAPI, 4′,6-diamidino-2-phenylindole.