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Figure 4 | Stem Cell Research & Therapy

Figure 4

From: Comparison of the neuropoietic activity of gene-modified versus parental mesenchymal stromal cells and the identification of soluble and extracellular matrix-related neuropoietic mediators

Figure 4

Comparison of SB623- and MSC-derived ECM in supporting nestin-positive cell growth. (A) Rat neural cells were grown for 5 days on MSC- or SB623-derived ECM and then immunostained for nestin (upper panel) and counterstained for nuclei (lower panel); magnification 100×. (B) Nestin-positive cells grown and stained as described in (A) were counted, and their numbers expressed as percentage of total nuclei. (C) Rat nestin expression in cells growing on MSC- or SB623-ECM was quantified by using qRT-PCR and normalized by the LDH activity released from either MSC- or SB623-ECM-producing cells during ECM preparation, correspondingly, to account for possible differences in cell numbers.

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Stem Cell Research & Therapy

ISSN: 1757-6512