Differentiated mDPSC produce central and peripheral nervous system markers. At day 11 of differentiation mDPSC express central and peripheral nervous system glial marker, S100 (A), and acetylcholine-specific enzyme, ChAT (B). mDPSC also show positive expression of GAD 65/67 (C) and vGlut2 (D) but not TH (E). Scale bar = 25 μm. ChAT, choline acetyltransferase; GAD65/57, glutamic acid decarboxylase 65/67; mDPSC, murine dental pulp stem cells; TH, tyrosine hydroxylase; vGlut2, vesicular glutamate transporter 2.