Figure 5

Differentiated mDPSC express voltage-gated Ca²⁺currents. A) Representative whole cell patch clamp recording of an isolated differentiated mDPSC with multiprocessor neuronal morphology showing typical voltage-gated Ca²⁺ current in response to increasing voltage steps; 500 ms voltage steps were applied from -50 to +50 mV in 10 mV increments. B) There is a two-fold increase in current amplitude in response to the addition of 10 mM Ba²⁺. C) The current–voltage relationship of voltage-dependent current from one representative cell with neuronal morphology showing sensitivity to 10 mM Ba²⁺ addition. The amplitude of the current was normalised to cell capacitance. D) Representative L-type Ca²⁺ currents recorded from undifferentiated mDPSC (UD, green) and differentiated DPSC (D, black) in the presence of 10 mM Ca²⁺ and following the addition of Ba²⁺ (D, red), in response to 100 ms voltage ramps from -120 to +120 mV. mDPSC, murine dental pulp stem cells.