Figure 3
From: Culture bag systems for clinical applications of adult human neural crest-derived stem cells
Bag-cultured ITSCs keep their potential to undergo mesodermal differentiation. (A) Schematic overview of directed osteogenic and adipogenic differentiation assays. (B) Representative phase contrast microscopy images (biological triplicate) revealing ALP-activity (left panel) as well as Alizarin Red S-(middle panel) and Von Kossa-positive (right panel) Ca2+-deposition of ITSCs cultivated under osteogenic differentiation conditions after bag culture. (C) RT-PCR analyses show expression of characteristic osteogenic markers osteocalcin and osteopontin in bag-cultured ITSCs after directed osteogenic differentiation. GAPDH served as loading control. (D) Representative phase contrast microscopy images (biological triplicate) exhibiting the presence of Oil Red O-positive lipid droplets within adipogenically differentiated ITSCs after bag-culture. ALP, alkaline phosphatase; ITSCs, inferior turbinate stem cells.