Figure 4
From: Culture bag systems for clinical applications of adult human neural crest-derived stem cells
ITSCs maintain neuronal differentiation capacity after bag culture. (A) Schematic overview of directed neuronal differentiation of ITSCs. (B) Representative immunocytochemical analyses (biological triplicate) showed coexpression of neuronal markers β-III-tubulin and MAP2 in bag-cultured ITSCs after four weeks of directed neuronal differentiation. (C) Schematic view on synaptic vesicle recycling assay. (D) Synaptic vesicle recycling assay revealed no FM1-43FX signal in undifferentiated ITSCs (upper panel), while bag-cultured ITSCs differentiated for 30 days were able to recycle synaptic vesicles, as visualized by FM1-43FX-labeling (lower panel). Representative images of biological triplicate. ITSCs, inferior turbinate stem cells; MAP2, microtubule-associated protein 2.