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Figure 1 | Stem Cell Research & Therapy

Figure 1

From: Functional differentiation of midbrain neurons from human cord blood-derived induced pluripotent stem cells

Figure 1

The utilization of dorsomorphin (DM) and SB 431542 (SB) in the hCBiPSCs differentiation process significantly improved neural conversion. Relative gene expression was measured by quantitative real-time PCR. (A) A marked increase in the expression of the neural stem cell markers Sox1 and Pax6 was observed during the first four days of differentiation by application of DM/SB (DIFF+) compared to cells differentiated without these molecules (DIFF-). (B) Downregulation of the pluripotency marker Oct4 was found as the maturation proceeded under both conditions. Midbrain regionalization, monitored by the expression of the midbrain marker FoxA2, was induced by PMA/FGF8 treatment (DIFF+) on day 4 of differentiation. A significantly higher FoxA2 level was observed in PMA/FGF8 treated cells compared to controls (DIFF-) from day 10 on. Values are calculated as means ± SEM. P-values (*P <0.05, **P <0.01, ***P <0.001) were determined using two-way ANOVA and Bonferroni posttest. ANOVA, analysis of variance; FGF8, fibroblast growth factor 8; hCBiPSCs, human cord blood induced pluripotent stem cells; PMA, purmorphamine; SEM, standard error of the mean.

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