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Figure 3 | Stem Cell Research & Therapy

Figure 3

From: Functional differentiation of midbrain neurons from human cord blood-derived induced pluripotent stem cells

Figure 3

hCBiPSCs differentiated into dopaminergic and GABAergic neurons. (A, E) After 40 days of in vitro differentiation (40 DIV) almost 60% of the cells were positive for the neuronal marker beta III tubulin (Tuj1) and 40% showed reactivity against MAP2 indicating more mature neurons. A minor cell population of roughly 13% was positive for the astrocytic marker GFAP (B, E). Approximately 17% of Tuj1-positive cells were double labeled with antibodies against the dopaminergic marker tyrosine hydroxylase (TH, C, E) and nearly 40% were immunopositive for GABA (D, E). Scale bars represent 50 μm. Values are calculated as means ± SEM. hCBiPSC, human cord blood induced pluripotent stem cells; SEM, standard error of the mean.

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