Figure 6

Differentiated hCBiPSC exhibited spontaneous synaptic activity. (A) Current traces recorded in whole-cell voltage-clamp mode from a single cell of the hCBiPSC line K2 under control conditions (top) and following application of the AMPA receptor blocker NBQX (10 μM, middle) or the GABA_A receptor blocker bicuculline (BIC, 10 μM, bottom). (B) Average frequency of spontaneous post-synaptic currents (PSCs). Application of BIC significantly reduced the PSC frequency to 17.0% (n = 5), NBQX just slightly depressed PSCs to 74.5% (n = 5). hCBiPSC, human cord blood induced pluripotent stem cells.