Figure 5

Inhibitors for NFκB p65 and ERK 1/2 suppress thrombin-stimulated FN secretion. A: MSCs were pretreated with EP or PD, followed by thrombin treatment with a dose of 4 U/ml. The cells were collected at the indicated time points for Western blotting. B: The supernatants were collected after MSCs were cultured for 48 hours in the presence or absence of thrombin (TH, 4 U/ml), EP or PD. The concentrations of FN were assessed by ELISA. **P <0.01 and *P <0.05 compared with control (CTR). ##P <0.01 compared with TH. The results were from three individual experiments. EP, Ethyl pyruvate; FN, Fibronectin; MSCs, Mesenchymal stem cells; NFκB, Nuclear factor kappa B; PD, PD98059.