Effects of hWJMSC-MVs on macrophage recruitment and the cytokine expression in injured kidneys. (A) Representative images of interstitial macrophages labeled with CD68 in the kidney on 24 h, 48 h and 2 weeks after reperfusion (the original magnification is 400×, Scale bar = 50 um). (B) Quantification of CD68+ macrophages/HPF in the kidney sections at different time points after reperfusion, all quantitative data are shown as mean ± SD of six kidney samples for each experimental condition (n = 6, *P <0.05, sham versus vehicle; #P <0.05, MVs versus vehicle; **P <0.05, sham versus MVs) (C) ELISA analysis of the anti-inflammatory cytokine IL-10 in injured kidney at 24 h and 48 h after reperfusion, (n = 3, #P <0.05, MVs versus vehicle). (D) ELISA analysis of the TNF-α in the injured kidney at 24 h and 48 h after reperfusion. All data are shown as mean ± SD of three kidney samples for each experimental condition. (n = 3, #P <0.05, MVs versus vehicle). HPF, high power fields; hWJMSC-MVs, human Wharton-Jelly MSCs derived microvesicles.