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Figure 7 | Stem Cell Research & Therapy

Figure 7

From: Shear stress-induced mechanotransduction protein deregulation and vasculopathy in a mouse model of progeria

Figure 7

Western blot and mRNA analysis of vimentin in the descending aortas after artificial shear stress. Vimentin expression in 12-month-old wild type (WT) and mLMNA+ descending aortas provided control values for a study that involved a 30-minute ex vivo exposure to high fluidic shear stress. In triplicate samples no difference was detected between WT and mLMNA+ control samples not exposed to shear stress (A). This image was the same figure as Figure 6A (12 m descending WT and mLMNA+). After exposure to shear stress, a 50% reduction in vimentin expression was demonstrated in mLMNA+ mice compared with WT mice (A). Vimentin mRNA expression in descending aortas of WT and mLMNA+ mice was obtained by RT-PCR analysis after exposure to shear stress (B).

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