Figure 7
From: Characterization of the radioresponse of human apical papilla-derived cells
Effect of irradiation on hard tissue-forming capacity in vivo in APDCs and PSFCs. (a) A typical example of hard tissue generation in vivo. (a) Generated bone-like hard tissue. Osteocyte-like cells (OC) embedded in the mineralized matrix and osteoblastic cells (OB) lining the surface of the hard tissue. HA, hydroxyapatite. (b) Immunofluorescence staining for osteocalcin (OCN). green: OCN; blue: nuclei. Scale: 100 μm. (b) A typical example of hard tissue generation in vivo in non-irradiated and irradiated cells. HA, hydroxyapatite. (c) Quantitative analysis of the generated hard tissue area in three independent samples. Results are expressed as means ± SE of five pieces of sections in each sample. *P < 0.05.