Skip to main content
Figure 2 | Stem Cell Research & Therapy

Figure 2

From: Inositol pyrophosphates mediate the effects of aging on bone marrow mesenchymal stem cells by inhibiting Akt signaling

Figure 2

IP7 synthesis and Akt activation in young and aged MSCs after hypoxia and serum deprivation (H/SD) injury. (A) HPLC profiles of inositol phosphates isolated from young and aged MSCs under normoxic or hypoxic conditions. (B) Quantitative analysis of IP7 production as a percentage of IP6 in MSCs. (C) Representative Western blots of p-Akt (T308), Akt, p-Bad, Bad, Bcl-2 and Bax expression in young and aged MSCs after H/SD. Semiquantitative analysis of p-Akt (D), p-Bad (E), Bcl-2 and Bax (F). Data are expressed as mean ± SEM. n = 5, *P <0.05, CPM, counts per minute. H/SD, hypoxia and serum deprivation; IP7, 5-diphosphoinositol pentakisphosphate; MSCs, mesenchymal stem cells; SEM, standard error of the mean.

Back to article page