Figure 2From: Inositol pyrophosphates mediate the effects of aging on bone marrow mesenchymal stem cells by inhibiting Akt signalingIP7 synthesis and Akt activation in young and aged MSCs after hypoxia and serum deprivation (H/SD) injury. (A) HPLC profiles of inositol phosphates isolated from young and aged MSCs under normoxic or hypoxic conditions. (B) Quantitative analysis of IP7 production as a percentage of IP6 in MSCs. (C) Representative Western blots of p-Akt (T308), Akt, p-Bad, Bad, Bcl-2 and Bax expression in young and aged MSCs after H/SD. Semiquantitative analysis of p-Akt (D), p-Bad (E), Bcl-2 and Bax (F). Data are expressed as mean ± SEM. n = 5, *P <0.05, CPM, counts per minute. H/SD, hypoxia and serum deprivation; IP7, 5-diphosphoinositol pentakisphosphate; MSCs, mesenchymal stem cells; SEM, standard error of the mean.Back to article page