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Figure 4 | Stem Cell Research & Therapy

Figure 4

From: Inositol pyrophosphates mediate the effects of aging on bone marrow mesenchymal stem cells by inhibiting Akt signaling

Figure 4

TNP decreased IP7 production and enhanced Akt activation in aged MSCs. (A) HPLC profiles of inositol phosphates isolated from aged MSCs with or without the IP6Ks inhibitor TNP. (B) Quantitative analysis of IP7 production as a percentage of IP6 in MSCs. Representative Western blots and semiquantitative analysis of the expressions of p-Akt (T308) (C), p-Bad (D), Bcl-2 and Bax (E) in aged MSCs after H/SD with or without TNP incubation. Data are expressed as mean ± SEM. n = 5, *P <0.05. CPM, counts per minute. H/SD, hypoxia and serum deprivation; IP6Ks, inositol hexakis phosphate kinases; IP7, 5-diphosphoinositol pentakisphosphate; MSCs, mesenchymal stem cells; SEM, standard error of the mean; TNP, N6-(p-nitrobenzyl) purine.

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