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Figure 5 | Stem Cell Research & Therapy

Figure 5

From: Inositol pyrophosphates mediate the effects of aging on bone marrow mesenchymal stem cells by inhibiting Akt signaling

Figure 5

TNP decreased apoptosis of aged MSCs. (A) Representative confocal microscopic images of TUNEL stains for the hypoxia-induced apoptosis in aged MSCs with or without the IP6Ks inhibitor TNP. (B) Apoptosis was also analyzed by flow cytometry after staining with Annexin V and PI. (C) Quantification of the TUNEL assay is presented as the percentage of apoptotic cells. (D) Quantification of the FACS analysis. Viable cells: Annexin V-/PI-; early apoptosis: Annexin V+/PI-; late apoptosis: V+/PI+; necrotic: V-/PI+. (E) Caspase-3 activity of MSCs in all groups. Data are expressed as mean ± SEM. n = 5, *P <0.05, # P <0.05 versus H/SD. FACS, fluorescence-activated cell sorting; H/SD, hypoxia and serum deprivation; IP6Ks, inositol hexakis phosphate kinases; MSCs, mesenchymal stem cells; SEM, standard error of the mean; TNP, N6-(p-nitrobenzyl) purine; TUNEL, terminal- deoxynucleotidyl transferase mediated nick end labeling.

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