Figure 5From: Inositol pyrophosphates mediate the effects of aging on bone marrow mesenchymal stem cells by inhibiting Akt signalingTNP decreased apoptosis of aged MSCs. (A) Representative confocal microscopic images of TUNEL stains for the hypoxia-induced apoptosis in aged MSCs with or without the IP6Ks inhibitor TNP. (B) Apoptosis was also analyzed by flow cytometry after staining with Annexin V and PI. (C) Quantification of the TUNEL assay is presented as the percentage of apoptotic cells. (D) Quantification of the FACS analysis. Viable cells: Annexin V-/PI-; early apoptosis: Annexin V+/PI-; late apoptosis: V+/PI+; necrotic: V-/PI+. (E) Caspase-3 activity of MSCs in all groups. Data are expressed as mean ± SEM. n = 5, *P <0.05, # P <0.05 versus H/SD. FACS, fluorescence-activated cell sorting; H/SD, hypoxia and serum deprivation; IP6Ks, inositol hexakis phosphate kinases; MSCs, mesenchymal stem cells; SEM, standard error of the mean; TNP, N6-(p-nitrobenzyl) purine; TUNEL, terminal- deoxynucleotidyl transferase mediated nick end labeling.Back to article page