Skip to main content
Figure 1 | Stem Cell Research & Therapy

Figure 1

From: Excision of viral reprogramming cassettes by Cre protein transduction enables rapid, robust and efficient derivation of transgene-free human induced pluripotent stem cells

Figure 1

Scheme of induced pluripotent stem cell generation and subsequent Cre protein transduction to obtain transgene-free induced pluripotent stem cells. (A) Reprogramming of human fibroblasts to induced pluripotent stem cells (iPSCs) via lentiviral vector STEMCCA. (B) Schematic representation of TAT-Cre treatment to obtain transgene-free iPSCs. (C) Schematic representation of the genomic locus before (top) and after (bottom) Cre-mediated recombination. LTR, long terminal repeat; PCR, polymerase chain reaction.

Back to article page

Stem Cell Research & Therapy

ISSN: 1757-6512