Figure 5
Quantification of neovessels in Matrigel plugs. Freshly isolated SVF cells, SVF-derived MSCs, and PB CD14+ monocytes, freshly isolated or after co-culture with SVF-derived MSCs in a transwell system, were individually embedded in Matrigel at 1 × 106 cells per implant. Matrigel explants were harvested 14 days after implantation and H&E-stained sections were used to enumerate blood vessels (A). The lumen area of newly formed vessels in Matrigel implants containing SVF-derived MSCs or SVF-isolated CD45+ or CD45- cells was determined. Vessel distribution indicates the percentage of vessels according to their lumen area, given as range of values (B). Results are expressed as the mean and SD of five Matrigel implants per test group. MSCs derived from three independent SVFs were used. *P < 0.0001 for differences between groups linked by the brackets.