Figure 1

MSCs obtained from human amniotic and chorionic membranes of term placentas. A) Inverted light microscope images of cells derived from three different placentas (P2, P10, P26) at different days from adhesion on culture plastics. Original magnification: 40X. B) Inverted light microscope images of P2, P10 and P26 placenta adherent cells subcultured in vitro. The micrograph is an example of a five-day culture at 6 pd (passage 4). Original magnification: 40X. C) FCM analysis of HLA-ABC and HLA-DR antigens in P2, P10 and P26 placenta cultured cells. A negative sample was incubated with the corresponding isotype. The cytograms shown are representative of three different experiments with similar results. FCM, flow cytometry; MSCs, mesenchymal stem cells; pd, population doubling.