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Figure 3 | Stem Cell Research & Therapy

Figure 3

From: Human placenta-derived neurospheres are susceptible to transformation after extensive in vitro expansion

Figure 3

Neural lineage induction and terminal differentiation. A) Secondary PD-neurospheres after four and seven days of culture in the non-adhesion conditions. LAN-5 and SK-N-SH NB cells were used as positive and negative controls, respectively. B) FCM analysis of the cell cycle related marker Ki67 in secondary PD-neurospheres after seven days of culture. The negative is the sample incubated with the corresponding isotype. The cytograms shown are representative of three different experiments with similar results. C) FCM analysis of the indicated neural stem cells markers in secondary PD-neurospheres after seven days of culture. The negative is the sample incubated with the correspondent isotype. The cytograms shown are representative of three different experiments with similar results. D) Levels of the indicated mRNA in secondary PD-neurospheres (black) after seven days of culture. The data are reported as the level of mRNA relative to the PD-MSCs (white) after five days of culture at 3.6 pd (passage 4) and are the means + SD (n = 3). Statistical significance: *P <0.05; **P <0.01; ***P <0.001. FCM, flow cytometry; PD-MSCs, placenta derived-mesenchymal stem cells; pd, population doubling; SD, standard deviation.

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