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Figure 2 | Stem Cell Research & Therapy

Figure 2

From: Gene markers of cellular aging in human multipotent stromal cells in culture

Figure 2

Gene filtration. (A) The mean difference between passage 7 and passage 3 versus the technical-variability cutoff point per probe for 34,555 probes. Blue probes (6,219) indicate that the differences between passages 7 and 3 are measurable. Blue probes were above the cutoff and used in further analysis, and black probes were below the cutoff and not used in further analysis. (B) Volcano plot of the mean difference between passages 7 and 3 versus –Log10 (P value). Blue probes (1,713) indicate that the differences between passages 7 and 3 are greater than the biologic variability. (C) Distribution of the A signals of the background spots with a mean and standard deviation of 9.042 ± 0.869. (D) MA plot of all the probes on the microarray. The blue spots represent the mean gene expression and A signal of the 911 probes at passage 7, and the red spots represent the mean gene expression and A signal at passage 3. The mean of the background (μ) and the mean of the background plus 1 standard deviation (μ + σ) are indicated by the red vertical lines.

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