Live cell imaging of adult human keratinocytes cultured in the presence or absence of Y-27632. A. Human foreskin keratinocytes at passage 6 were plated in triplicate in the presence or absence of Y-27632. Still images from live movies of each culture condition are shown at the times indicated to represent the confluence of the colonies. The corresponding movies are available as Supplementary data. B. Growth curves were calculated using time-lapse photography to measure confluence of cultures for up to six days (by Incucyte ZOOM software).