Angiogenic potential of immortalized hASCs. Conditioned media from hASCs-M and hASCs-T (passages 5-13-21), hASCs-TS and hASCs-TE (passages 16-22-38) were collected and subsequently analyzed by ELISA assay to evaluate the concentration of HGF and VEGF. For each sample analyzed, HGF and VEGF secretion were normalized by the total number of cells and expressed as pg/105 cells. A) The HGF secretion was significantly decreased in hASC-TS cell lines and increased in hASC-TE cell lines. B) The VEGF secretion was similar in hASC-M and hASC-TE cell lines but significantly decreased in hASC-TS cell lines. Each histogram represents the mean ± SD of four different experiments. *P <0.05 vs hASC-M protein levels.