Figure 4

Migration assay. a) Migratory capacity of the ADSCs (black bar) and A431-SCCs (red bar) alone and in co-culture, measured as the level of optical density at 560 nm, with a standard deviation (SD). When co-cultured with A431-SCCs (black bar with red checkerboard pattern) ADSCs showed a significantly higher migration as in mono-culture (P = 0.014). Co-culture of A431-SCCs with ADSCs (red bars with black diagonal slashes) does not lead to a significant change in the migratory properties of A431-SCCs (P >0.05). b) Migratory capacity of the ADSCs (black bar) and pSCCs (yellow bar) alone and in co-culture, measured as the level of optical density at 560 nm, with a SD. When co-cultured with pSCCs (black bar with yellow checkerboard pattern) ADSCs showed a higher migration as in mono-culture (P >0.05). Co-culture of pSCCs with ADSCs (yellow bars with black diagonal slashes) resulted in a significantly increased migration of pSCCs (P = 0.009). ADSC, adipose tissue derived stem cells; pSCCs, primary squamous cell carcinoma cells; SCCs, squamous cell carcinoma cells.