Figure 5

Invasion assay. a) Invasive behavior of ADSCs (black bar) and A431-SCCs (red bar) alone and in co-culture, measured as the level of optical density at 560 nm with a standard deviation (SD). When co-cultured with A431-SCCs (black bar with red checkerboard pattern) ADSCs showed a significantly higher invasive capacity as in mono-culture (P = 0.014). Co-culture of SCCs with ADSCs (red bars with black diagonal slashes) also leads to a significant increase in the invasive behavior of A431-SCCs (P <0.001). b) Invasive behavior of ADSCs (black bar) and pSCCs (yellow bar) alone and in co-culture, measured as the level of optical density at 560 nm, with a SD. When co-cultured with pSCCs (black bar with yellow checkerboard pattern) ADSCs did not significantly change their invasive capacity compared to mono-culture. Co-culture of pSCCs with ADSCs (yellow bars with black diagonal slashes), however, leads to a significant increase in the invasive behavior of pSCCs (P = 0.013). ADSC, adipose tissue derived stem cells; pSCCs, primary squamous cell carcinoma cells; SCCs, squamous cell carcinoma cells.