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Table 1 Changes in the gene expression levels of ADSCs and A431-SCCs in co-culture compared to mono-culture

From: Alterations of gene expression and protein synthesis in co-cultured adipose tissue-derived stem cells and squamous cell-carcinoma cells: consequences for clinical applications

Gene Fold ADSC mono-/co-culture Up-/down-regulation Main impact of gene product
Part 1. Changes in the gene expression of ADSCs
E-Cadherin n.d. EMT, i, m, ms
N-Cadherin 1.0 (0.0) EMT
CCL2 9.4 (5.0) a, i, m, ms, p, EMT
CCL7 11 (3.8) a, i, m, ms, p
CCL8 42 (15) a, i, m, p
CCL13 8.0 (0.0) a, i, m, p
CCL20 32 (23) im
CSF-2 38 (20) a, i, m
CSF-3 258 (2.3) im
CXCL1 54 (16) a, i, m, ms, p
CXCL2 16 (0.0) a, i, m
CXCL3 27 (7.8) a, i, m
CXCL5 9.4 (5.0) a, i, m
CXCL6 129 (0.2) a, i, m, ms, p
CXCL11 4.0 (0.0) a, i, m
CXCL12 12 (5.7) a, i, m
IL-1α 12 (5,6) a, ms
IL-1β 119 (100) a, ms
IL-6 88 (84) p
IL-8 29 (25) a
LYPD3 6.1 (2.9) i, ms
MMP-3 108 (32) m, i, ms
NR4A3 4.7 (2.8) p
PTGS2 74 (39) a, i, im, m, ms, p
TLR-2 4.6 (2.5) a, m, i
WISP-1 6.1 (2.9) m, im
Gene Fold A431-SCC mono-/co-culture Up-/down-regulation Main impact of gene or gene product
Part 2. Changes in the gene expression of A431-SCCs
E-Cadherin * ↓↓ EMT, i, m, ms
N-Cadherin 98 (2.3) EMT
CXCL9 4.4 (0.2) a
CXCL10 4.8 (0.1) a
CTKS 4.1 (2.9) m, i, ms
FN1 2.5 (1.3) m, i
HGF 20 (12) a, m, i,
ITGB3 10 (5.1) m, i, ms
MMP-1 9.4 (5.1) m, i, ms
MMP-2 3.1 (1.5) m, i, ms
MMP-3 191 (89) m, i, ms
MMP-7 4.8 (2.4) m, i, ms
MMP-9 3.4 (0.2) m, i, ms
PTGS2 2.7 (0.8) a, i, im, m, ms, p
TWIST-1 11 (3.8 ) ms
  1. *In co-culture with ADSCs E-Cadherin expression is down-regulated in primary SCC to undetectable levels.
  2. GUSB was used as referring housekeeping-gene. Except for E- and N-Cadherin only changes of 2.5-fold or higher are displayed. Part 1 displays the changes in the gene expression levels of ADSCs. Part 2 shows the changes in the gene expression levels of A431-SCCs. Arrows mark an up- (↑) or down-regulation (↓) of the gene expression compared to the referring mono-culture. Abbreviations of main impact: a, angiogenesis; i, invasion, im, impaired anti-tumor response; m, migration; ms, metastasis; p, proliferation.;Furthermore a mild down-regulation of E2F1 (3.2-fold), CCND1, CCND3, CYCS and DVL1 (2.7-fold) and SPP1 (2.6-fold) could be determined.