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Table 1 Changes in the gene expression levels of ADSCs and A431-SCCs in co-culture compared to mono-culture

From: Alterations of gene expression and protein synthesis in co-cultured adipose tissue-derived stem cells and squamous cell-carcinoma cells: consequences for clinical applications

Gene

Fold ADSC mono-/co-culture

Up-/down-regulation

Main impact of gene product

Part 1. Changes in the gene expression of ADSCs

E-Cadherin

n.d.

→

EMT, i, m, ms

N-Cadherin

1.0 (0.0)

→

EMT

CCL2

9.4 (5.0)

↑

a, i, m, ms, p, EMT

CCL7

11 (3.8)

↑

a, i, m, ms, p

CCL8

42 (15)

↑

a, i, m, p

CCL13

8.0 (0.0)

↑

a, i, m, p

CCL20

32 (23)

↑

im

CSF-2

38 (20)

↑

a, i, m

CSF-3

258 (2.3)

↑

im

CXCL1

54 (16)

↑

a, i, m, ms, p

CXCL2

16 (0.0)

↑

a, i, m

CXCL3

27 (7.8)

↑

a, i, m

CXCL5

9.4 (5.0)

↑

a, i, m

CXCL6

129 (0.2)

↑

a, i, m, ms, p

CXCL11

4.0 (0.0)

↑

a, i, m

CXCL12

12 (5.7)

↑

a, i, m

IL-1α

12 (5,6)

↑

a, ms

IL-1β

119 (100)

↑

a, ms

IL-6

88 (84)

↑

p

IL-8

29 (25)

↑

a

LYPD3

6.1 (2.9)

↑

i, ms

MMP-3

108 (32)

↑

m, i, ms

NR4A3

4.7 (2.8)

↑

p

PTGS2

74 (39)

↑

a, i, im, m, ms, p

TLR-2

4.6 (2.5)

↑

a, m, i

WISP-1

6.1 (2.9)

↑

m, im

Gene

Fold A431-SCC mono-/co-culture

Up-/down-regulation

Main impact of gene or gene product

Part 2. Changes in the gene expression of A431-SCCs

E-Cadherin

*

↓↓

EMT, i, m, ms

N-Cadherin

98 (2.3)

↑

EMT

CXCL9

4.4 (0.2)

↓

a

CXCL10

4.8 (0.1)

↓

a

CTKS

4.1 (2.9)

↑

m, i, ms

FN1

2.5 (1.3)

↑

m, i

HGF

20 (12)

↑

a, m, i,

ITGB3

10 (5.1)

↑

m, i, ms

MMP-1

9.4 (5.1)

↑

m, i, ms

MMP-2

3.1 (1.5)

↑

m, i, ms

MMP-3

191 (89)

↑

m, i, ms

MMP-7

4.8 (2.4)

↑

m, i, ms

MMP-9

3.4 (0.2)

↓

m, i, ms

PTGS2

2.7 (0.8)

↑

a, i, im, m, ms, p

TWIST-1

11 (3.8 )

↑

ms

  1. *In co-culture with ADSCs E-Cadherin expression is down-regulated in primary SCC to undetectable levels.
  2. GUSB was used as referring housekeeping-gene. Except for E- and N-Cadherin only changes of 2.5-fold or higher are displayed. Part 1 displays the changes in the gene expression levels of ADSCs. Part 2 shows the changes in the gene expression levels of A431-SCCs. Arrows mark an up- (↑) or down-regulation (↓) of the gene expression compared to the referring mono-culture. Abbreviations of main impact: a, angiogenesis; i, invasion, im, impaired anti-tumor response; m, migration; ms, metastasis; p, proliferation.;Furthermore a mild down-regulation of E2F1 (3.2-fold), CCND1, CCND3, CYCS and DVL1 (2.7-fold) and SPP1 (2.6-fold) could be determined.