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Figure 3 | Stem Cell Research & Therapy

Figure 3

From: Under a nonadherent state, bone marrow mesenchymal stem cells can be efficiently induced into functional islet-like cell clusters to normalize hyperglycemia in mice: a control study

Figure 3

Dithizone staining and quantitative polymerase chain reaction analysis of islet-like cell clusters from bone marrow mesenchymal stem cells of human first-trimester abortus. (A) Islet-like cell clusters were positive for dithizone staining in the nonadherent induction group (a) and the adherent induction group (b), similar to those of fetal pancreatic islets (c), whereas non-induced bone marrow mesenchymal stem cells of human first-trimester abortus were negative for dithizone (d). (B) Expression levels of Pdx1, Ngn3, Pax4, NeuroD1, Nkx2.2, Nkx6.1, PCSK1, insulin, glucagon, SST and PP genes in fluorescent quantitative reverse transcriptase-polymerase chain reaction test. The islet-like cell clusters expressed significantly more Ngn3 and Pax4, and significantly less insulin, glucagon, SST and PP than fetal pancreatic islets (P < 0.01, n = 10). The expressions of all the genes were not different between the nonadherent induction group and the adherent induction group (P > 0.05, n = 10).

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Stem Cell Research & Therapy

ISSN: 1757-6512