Gene | Primer sequence (5′ to 3′) | Size of PCR product (base pairs) | Annealing temperature (°C) | Cycle number |
---|---|---|---|---|
18SrRNA | Forward: CGGCTACCACATCCAAGGAA | 187 | 62 | 40 |
Reverse: GCTGGAATTACCGCGGCT | ||||
Pdx1 | Forward: TTCCGGAAGAAAAAGAGCCA | 129 | 62 | 40 |
Reverse: AAACAGGTCCCAAGGTGGAGT | ||||
Ngn3 | Forward: CGCAATCGAATGCACAACCT | 135 | 62 | 40 |
Reverse: TTGAGTCAGCGCCCAGATGTA | ||||
Pax4 | Forward: ATTCAGTGGCCCGTGGAAA | 102 | 62 | 40 |
Reverse: TCTCTTGCCGACGCCATTT | ||||
NeuroD1 | Forward: TCTTTCAAACACGAACCGTCC | 103 | 62 | 40 |
Reverse: AGATTGATCCGTGGCTTTGG | ||||
Nkx2.2 | Forward: GCCACGAATTGACCAAGTGAA | 103 | 62 | 40 |
Reverse: ATGTCCTTGACCGAAAACCCC | ||||
Nkx6.1 | Forward: ACACGAGACCCACTTTTTCCG | 102 | 62 | 40 |
Reverse: AATAGGCCAAACGAGCCCTCT | ||||
PCSK1 | Forward: TCCTCTTTTGCGCTTGGTGT | 139 | 62 | 40 |
Reverse: TGACCCAAAAGGTCATAGCCC | ||||
Insulin | Forward: GCGTGGCATTGTGGAACAA | 123 | 62 | 40 |
Reverse: CCATCTCTCTCGGTGCAGGA | ||||
Glucagon | Forward: ACATTGCCAAACGTCACGATG | 103 | 62 | 40 |
Reverse: GCAATGAATTCCTTGGCAGCT | ||||
SST | Forward: GAAGCAGGAACTGGCCAAGTA | 118 | 62 | 40 |
Reverse: CCTCATTTCATCCTGCTCAGC | ||||
PP | Forward: ACAATGCCACACCAGAGCAGA | 110 | 62 | 40 |
 | Reverse: GGCCAGCGTGTCCTCTTTG |  |  |  |