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Table 1 Human gene-specific primers and quantitative polymerase chain reaction conditions

From: Under a nonadherent state, bone marrow mesenchymal stem cells can be efficiently induced into functional islet-like cell clusters to normalize hyperglycemia in mice: a control study

Gene

Primer sequence (5′ to 3′)

Size of PCR product (base pairs)

Annealing temperature (°C)

Cycle number

18SrRNA

Forward: CGGCTACCACATCCAAGGAA

187

62

40

Reverse: GCTGGAATTACCGCGGCT

Pdx1

Forward: TTCCGGAAGAAAAAGAGCCA

129

62

40

Reverse: AAACAGGTCCCAAGGTGGAGT

Ngn3

Forward: CGCAATCGAATGCACAACCT

135

62

40

Reverse: TTGAGTCAGCGCCCAGATGTA

Pax4

Forward: ATTCAGTGGCCCGTGGAAA

102

62

40

Reverse: TCTCTTGCCGACGCCATTT

NeuroD1

Forward: TCTTTCAAACACGAACCGTCC

103

62

40

Reverse: AGATTGATCCGTGGCTTTGG

Nkx2.2

Forward: GCCACGAATTGACCAAGTGAA

103

62

40

Reverse: ATGTCCTTGACCGAAAACCCC

Nkx6.1

Forward: ACACGAGACCCACTTTTTCCG

102

62

40

Reverse: AATAGGCCAAACGAGCCCTCT

PCSK1

Forward: TCCTCTTTTGCGCTTGGTGT

139

62

40

Reverse: TGACCCAAAAGGTCATAGCCC

Insulin

Forward: GCGTGGCATTGTGGAACAA

123

62

40

Reverse: CCATCTCTCTCGGTGCAGGA

Glucagon

Forward: ACATTGCCAAACGTCACGATG

103

62

40

Reverse: GCAATGAATTCCTTGGCAGCT

SST

Forward: GAAGCAGGAACTGGCCAAGTA

118

62

40

Reverse: CCTCATTTCATCCTGCTCAGC

PP

Forward: ACAATGCCACACCAGAGCAGA

110

62

40

 

Reverse: GGCCAGCGTGTCCTCTTTG

   
  1. PCR, polymerase chain reaction.