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Figure 1 | Stem Cell Research & Therapy

Figure 1

From: Lipopolysaccharide differentially affects the osteogenic differentiation of periodontal ligament stem cells and bone marrow mesenchymal stem cells through Toll-like receptor 4 mediated nuclear factor κB pathway

Figure 1

TLR4 expression of PDLSCs and BMMSCs with or without LPS treatment. (A) Relative gene expression of TLRs (TLR1 to TLR10) of PDLSCs and BMMSCs was analyzed by qRT-PCR. Relative gene expression of TLRs was determined based on the threshold cycle (CT) values. The expression levels of the target genes were normalized to that of the housekeeping gene β-actin. PDLSCs (*P <0.05 versus PDLSCs, n = 3), BMMSCs (n = 3). (B) Western blot analysis showed the protein expression of TLR4, and β-actin was used as the internal control. (C) Relative intensity of the tested protein was quantitatively analyzed using the Adobe Photoshop CS2 software. Data represent the means ± SD (n = 3). BMMSCs, bone marrow mesenchymal stem cells; LPS, lipopolysaccharide; PDLSCs, periodontal ligament stem cells; SD, standard deviation; TLR4, Toll-like receptor 4.

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Stem Cell Research & Therapy

ISSN: 1757-6512