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Figure 3 | Stem Cell Research & Therapy

Figure 3

From: Lipopolysaccharide differentially affects the osteogenic differentiation of periodontal ligament stem cells and bone marrow mesenchymal stem cells through Toll-like receptor 4 mediated nuclear factor κB pathway

Figure 3

Anti-TLR4 antibody or PDTC effectively blocks the activated NF-κB pathway activated by LPS. (A) The TLR4 antagonist anti-TLR4 antibody (0.5 μg/ml) and the NF-κB inhibitor PDTC (40 ng/ml) were incubated with PDLSCs or BMMSCs, which were stimulated by LPS. After 72 hours, western blot showed that the expression of phospho-NFκBp65 was reduced. β-actin was used as the internal control. (B, C) The relative intensity of the tested protein was quantitatively analyzed using Adobe Photoshop CS2 software. Data represent the means ± SD. * P <0.05 (n = 3). BMMSCs, bone marrow mesenchymal stem cells; LPS, lipopolysaccharide; NF-κB, nuclear factor κB; PDLSCs, periodontal ligament stem cells; PDTC, pyrrolidinedithiocarbamate; SD, standard deviation; TLR4, Toll-like receptor 4.

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Stem Cell Research & Therapy

ISSN: 1757-6512