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Figure 7 | Stem Cell Research & Therapy

Figure 7

From: Lipopolysaccharide differentially affects the osteogenic differentiation of periodontal ligament stem cells and bone marrow mesenchymal stem cells through Toll-like receptor 4 mediated nuclear factor κB pathway

Figure 7

The number of osteoclasts does not change after anti-TLR4 antibody or PDTC treatment. (A) TRAP staining showed no significant differences in the number of osteoclasts in the alveolar bone among the saline, LPS, LPS + anti-TLR4 antibody and LPS + PDTC groups. Arrowheads, TRAP-positive osteoclasts (purple cells). (B) Quantification of TRAP-positive osteoclasts. Integrated intensity was measured by Image-Pro Plus 6.0. Scale bar: 50 μm. Data represent the means ± SD (n = 3). LPS, lipopolysaccharide; PDTC, pyrrolidinedithiocarbamate; SD, standard deviation; TLR4, Toll-like receptor 4; TRAP, tartrate-resistant acid phosphatase.

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