Figure 2From: Testicular tissue cryopreservation and spermatogonial stem cell transplantation to restore fertility: from bench to bedsideTesticular sperm viability after freeze and thaw. Live sperms were stained with Calcein (green fluorescent). (A) Bright field; (B) fluorescent; (C) merged. A LIVE/DEAD Sperm Viability Kit (L-7011 Invitrogen, Life Technologies Ltd, Paisley, UK) was used for staining thawed testicular sperms. Testicular tissue for research was obtained from transplant donors through the National Disease Research Interchange.Back to article page