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Table 1 Strategies safeguarding pluripotent stem cell therapy against iatrogenic cancerogenesis

From: ‘Above all, do no harm’: safeguarding pluripotent stem cell therapy against iatrogenic tumorigenesis

Authors Stem cells Key reagent Procedure Mechanism
Fong et al.[28] Human embryonic Mouse monoclonal antibodies anti-SSEA-4, anti-TRA-1-60 FACS, MACS Depletion of SSEA-4+, TRA-1-60 + hESCs
Tang et al.[30] Human embryonic Mouse monoclonal antibodies anti-SSEA-5, CD9, CD90 or anti-SSEA-5, CD50, CD200 FACS Depletion of SSEA-5+ hESCs
Human pluripotent induced
Ben-David et al.[31] Human embryonic Mouse monoclonal antibody anti-claudin-6 FACS Depletion of claudin-6+ hESCs
Killing by toxins linked to antibodies
Human induced pluripotent
King et al.[29] Human embryonic Mouse monoclonal antibodies anti-SSEA-4, anti-TRA-1-60, anti-FGFR-1, anti-CD133, anti-CD13519 FACS Separation of fate-specific subpopulations of GFP + hESCs
Malecki [11] Human autologous induced pluripotent Synthetic antibodies anti-SSEA-4, anti-TRA-1-60 MACS, FACS Depletion of SSEA-4+, TRA-1-60+ non-differentiating hiPSCs
Gerrard et al.[32] Human embryonic Fusion protein expressed from OCT4-EGFP Transfection with lentivirus vector Monitoring and selection of differentiating cells
Eiges et al.[33] Human embryonic Fusion protein expressed from Rex1 (murine promoter)-EGFP Electroporation LipofectAMINE, FuGENE, ExGen500 Monitoring and selection of undifferentiated cells
Nishimori et al.[16] Murine induced pluripotent Fusion protein expressed from NANOG-GFP Selection in embryoid bodies Discarding undifferentiated cells from EBs
van Laake et al.[34] Murine induced pluripotent Fusion protein expressed from NKX2-5-EGFP FACS Selection of differentiating cells
Chung S et al.[35] Murine embryonic Fusion protein expressed from SOX1-GFP FACS Selection of differentiating cells
Malecki et al.[10] Human autologous induced pluripotent Fusion fluorescent proteins expressed under promoters for OCLN5, CLDN5, TJP1, PECAM1, CDH5, CTNNB Transfection with synthetic antibody-guided vector Monitoring and selecting of differentiating cells
Choo et al.[36] Human embryonic Mouse monoclonal antibody anti-PODXL Antibody dissolved in media Killing undifferentiated cells
Schriebl et al.[37] Human embryonic Mouse monoclonal antibody anti-PODXL Antibody dissolved in media Sorting of undifferentiated cells + killing with toxic antibody
Lim et al.[38] Human embryonic ScFv 84-HTH antibody fragment anti- PODXL Antibody dissolved in media Killing undifferentiated cells in EBs prior to transplantation
Ben-David et al.[39] Human induced pluripotent PluriSIn#1 Reagent dissolved in media Inhibition of SCD1 leading to UPR, ER stress, and apoptosis
Conesa et al.[40] Murine embryonic Benzethonium chloride, methylbenzethonium chloride Reagent dissolved in media Mitochondrial membrane permeability, apoptosis
Human induced pluripotent
Lee et al.[41] Human embryonic Human induced pluripotent Quercetin, YM155 Reagent dissolved in media Mitochondria-mediated selective cell death
Vazquez-Martin et al.[42] Murine induced pluripotent Metformin Reagent dissolved in media Apoptosis
Bieberich et al.[43] Human embryonic Human induced pluripotent S18 (N-oleoyl serinol) Reagent dissolved in media Apoptosis
Schuldiner et al.[44] Human embryonic HSV-TK under PGK promoter + GCV Transfection with plasmid in ExGen500 Apoptosis
Hara et al.[45] Human embryonic HSV-TK under OCT4 promoter + GCV Transfection with viral vector Apoptosis
Rong et al.[46] Human embryonic HSV-TK gene into NANOG locus + GCV Homologous recombination Apoptosis
Cheng et al.[47] Human embryonic HSV-δTK under mouse NANOG or EF1α promoter + GCV Transfection with lentiviral vector Apoptosis
Human induced pluripotent
Chen et al.[48] Murine undifferentiated pluripotent stem Recombinant cytosine deaminase under mouse NANOG or EF1α promoter + 5-fluorocytosine Transfection with lentiviral vector Apoptosis
Malecki et al.[50] Human autologous induced pluripotent Human recombinant DNASE1, DNASE1L3, DNASE2, DFFB under POLA1 promoter Transfection with anti-SSEA-4-guided vector Apoptosis + necrosis
  1. EB, embryoid body; EGFP, enhanced green fluorescent protein; ER, endoplasmic reticulum; FACS, fluorescence activated cell sorting; FGFR, fibroblast growth factor receptor; GCV, ganciclovir; GFP, green fluorescent protein; hESC, human embryonic stem cell; hiPSC, human induced pluripotent stem cell; HSV, herpes simplex virus; MACS, magnetic activated cell sorting; SCD1, stearoyl-CoA desaturase 1; SSEA, stage specific embryonic antigen; TK, thymidine kinase; TRA, tumor related antigen; UPR, unfolded protein response.