Table 1 Primer pairs for SYBR Green analysis
From: Substrate and strain alter the muscle-derived mesenchymal stem cell secretome to promote myogenesis
Gene | Forward | Reverse |
|---|---|---|
Myf5 | TGAAGGATGGACATGACGGACG | TTGTGTGCTCCGAAGGCTGCTA |
MyoD | TACCCAAGGTGGAGATCCTG | CATCATGCCATCAGAGCAGT |
Myogenin | GAGACATCCCCCTATTTCTACCA | GCTCAGTCCGCTCATAGCC |
Pax7 | CTGGATGAGGGCTCAGATGT | GGTTAGCTCCTGCCTGCTTA |
L32 | TCCACAATGTCAAGGAGCTG | ACTCATTTTCTTCGCTGCGT |
COL1α2 | GCAGGTTCACCTACTCTGTCCT | CTTGCCCCATTCATTTGTCT |
Laminin α1 | CAGCGCCAATGCTACCTGT | GGATTCGTACTGTTACCGTCACA |
Laminin α2 | TCCCAAGCGCATCAACAGAG | CAGTACATCTCGGGTCCTTTTTC |
GM-CSF | ACCACCTATGCGGATTTCAT | TCATTACGCAGGCACAAAAG |
HGF | ATGTGGGGGACCAAACTTCTG | GGATGGCGACATGAAGCAG |