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Figure 3 | Stem Cell Research & Therapy

Figure 3

From: Osteoarthritic cartilage explants affect extracellular matrix production and composition in cocultured bone marrow-derived mesenchymal stem cells and articular chondrocytes

Figure 3

Quantification of collagens I, II, and III and glycosaminoglycan (GAG) in fibrin gel lysates. Protein synthesis of collagens (A) I, (B) II, (C) III, and (D) GAG in cell lysates after 28 days of culture. Bone marrow-derived stem cells (BMSCs) (white bars), mixed cultures (BMSCs and chondrocytes in a ratio of 1:1, light grey bars), or chondrocytes (dark grey bars) were kept as monocultures (F, bars with pattern) or as co- and tri-cultures with osteoarthritis cartilage explants (FC, blank bars) in chondrogenic medium. (A, B) Collagens I and II were quantified with enzyme-linked immunosorbent assay or (C) for collagen III by densitometrically evaluated dot-blot analysis including a recombinant collagen III standard curve. (D) GAG concentration in cell lysates was quantified by a dimethylmethylene blue assay including a chondroitin sulfate standard curve. Results are presented as mean with standard deviation. *P <0.05; **P <0.01. N = 7. Ch, chondrocytes.

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Stem Cell Research & Therapy

ISSN: 1757-6512