Figure 6

Quantification of cytokines and glycosaminoglycan (GAG) in culture supernatants. Analysis of supernatants at days 7 and 28 of bone marrow-derived stem cells (BMSCs) (white bars), mixed cultures (BMSCs and chondrocytes in a ratio of 1:1, light grey bars), or chondrocytes (dark grey bars) monocultured (F, bars with pattern) or co- tri-cultured with osteoarthritis cartilage (FC, blank bars). Total amount of cytokines (A) interleukin-1 beta (IL-1β), (B) IL-6, and (C) IL-8 was quantified by antigen-specific enzyme-linked immunosorbent assays. (D) GAG released into the supernatant was quantified by dimethylmethylene blue (DMMB) assay. Results are presented as mean with standard deviation. *P <0.05; **P <0.01; ***P <0.001. N = 6. Ch, chondrocytes.