Figure 8

Stimulation of fibrin gel monocultures with interleukin-1 beta (IL-1β), IL-6, or IL-8. Collagen gene expression of (A) IL-1β, (B) IL-6, and (C) IL-8 stimulated monocultured cells. Bone marrow-derived stem cells (BMSCs) (white bars), mixed cultures (BMSCs plus chondrocytes 1:1, light grey bars), or chondrocytes (dark grey bars) were embedded in fibrin gel and were stimulated with 5 ng/mL IL-1β, 5 ng/mL IL-6, or 10 ng/mL IL-8 daily for 7 days in chondrogenic medium containing dexamethasone and transforming growth factor-beta 3 (TGF-β3). Cultures were analyzed for gene expression of COL1A1, COL2A1, COL3A1, and COL10A1 at day 7 (blank bars) and day 28 (bars with dots). Results are presented as mean with standard deviation. *P <0.05; **P <0.01; ***P <0.001. N = 7. Cal, calibrator which represent the unstimulated monocultures; Ch, chondrocytes.