Figure 7

Single-cell transcriptional analysis of CD45^-/CD31^-/CD34⁺ASCs. (A) Hierarchical clustering of cells from wild-type (WT; left), db/db diabetic (DM2; middle), and STZ-induced diabetic (DM1; right) mice. Gene expression is presented as fold change from median on a color scale from yellow (high expression, 32-fold above median) to blue (low expression, 32-fold below median). See Additional file2: Figure S1 for complete dataset. (B) Differentially expressed genes between wild-type (WT) and diabetic (db/db [DM2] or STZ [DM1]) cells identified by using non-parametric two-sample Kolmogorov-Smirnov testing. Twenty-one genes exhibited significantly different (P <0.01 following Bonferroni correction for multiple comparisons) distributions of single-cell expression between populations; six are illustrated here by using median-centered Gaussian curve fits. The left bar for each panel represents the fraction of qPCR that failed to amplify in each group. Curves and p values for each gene are shown only for those diabetic groups significantly different from wild-type cells. (C) wild-type, (D) DM2, and (E) DM1 cells based on the expression patterns of all 71 genes (k = 3). (F) Pie charts representing the fraction of ASCs comprising each cluster (WT [red], DM2 [green], and DM1 [blue]). (G) List of selected cluster 3-defining genes.