Morphologic and multipotential characterization of cardiac mesenchymal stem cells (CMSCs). (A) Phase contrast microscopy of CMSCs shows a fibroblast-like morphology in culture. (B-D) CMSCs were submitted to differentiation protocols into mesenchymal lineages. Cell differentiation was confirmed by positive staining with Oil red for adipocytes (B), Alizarin red for osteocytes (C), and Alcian blue for chondrocytes (D). Magnification: 20×. (E) Flow cytometry analysis of CMSCs shows the percentages of CD29, CD90, CD45, Sca-1, CD44, CD105, CD117, and CD34 at passage 8.